Reactive IgA antibodies by direct immunofluorescence in a skin biopsy after being in saline in room temperature for 14 days

    Imagine this scenario .. the dermatologist biopsies a patient. He obtains two biopsies and sends them to the lab with the clinical impression being "dermatitis herpetiformis" (DH). The lab [mishandles the specimen] and forgets the fresh biopsy sitting in saline on the bench for 14 (yes, fourteen) days (!). The biopsy is discovered and is then accessioned and then shipped to another lab for "cutting" (for whatever reason). The other lab discovers the mishandling of the specimen and snap freezes the biopsy and returns it to the original lab frozen. You are the original lab and you now have that biopsy.

    Would you or would you not perform direct immunofluorescence (DIF) on this mishandled biopsy?

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