Discussion Workshop: Imaging Cell Migration in 3D
his event is a discussion workshop, focused on 3D imaging of cell migration. The aim is to bring together scientists to discuss important areas of work in this field. It offers participants a chance to explore aspects of the live cell imaging with the experts during round table and panel discussions.
Meeting Chair: Dr. Rainer Heintzmann, Friedrich-Schiller-Universität Jena, Germany
This event has CPD accreditation
On registration please submit your questions to the panel that will be asked by the chair on the day of the event
9:00 – 9:30 Registration
9:30 – 9:35 Introduction by Meeting Coordinator
9:30 – 9:40 Introduction by the Chair: Dr Rainer Heintzmann
Institute of Physical Chemistry, Friedrich-Schiller-Universität Jena, Germany
Talks by Invited Experts:
9:40 – 10:00 High Resolution Imaging in Deep Tissue
Dr Rainer Heintzmann
Institute of Physical Chemistry, Friedrich-Schiller-Universität Jena, Germany
10:00 – 10:20 Imaging Cell Adhesions in 3D Matrices
Kings College London, UK
Cell migration is a vital process involved in normal human development, wound healing and inflammatory responses. However, many pathological states such as cancer metastasis, developmental defects and healing abnormalities are as a result of a dysregulation in the control of cell motility. Research in our lab is directed towards understanding the molecular mechanisms that regulate adhesion, polarisation and migration in adherent cells in a number of different contexts. We use a number of state-of-the-art microscopy techniques to tackle these fundamental questions, and to allow us to analyse protein dynamics and interactions within intact cells in 3D environments. Unravelling the complex process of converting signals from the extracellular environment to drive changes in cell migration is important to understanding the basis of many different diseases.
10:20 – 10:40 Imaging Cellular Invasion: Role of the Cell Surface Collagen Degrading Enzyme
Kennedy Institute of Rheumatology, Imperial College London, UK
ECM plays a major role to maintain the architecture of tissues, to provide survival signals and differentiation signals to cells, to provide growth factor pools and scaffoldings for migration. On the other hand, ECM is also a physical barrier for migrating cells in tissues and needs to be degraded in order for cells to migrate through. Because cells require ECM as scaffolding, degradation of the barrier ECM needs to occur specifically at the direction of the migration. To degrade ECM barrier, cells utilise proteinases, and one of the plasma membrane-bound metalloproteinase, membrane-type 1 matrix metalloproteinase (MT1-MMP) is thought to be a critical enzyme for this process. MT1-MMP is regulated at the multiple steps, but homodimerization is an essential step to degrade collagen on the cell surface. By imaging this dimerization event in live cells using fluorescence resonance energy trasnfer, we found that MT1-MMP activity is regulated spatiotemporal manner during invasion.
10:40 – 11:00 Mid-morning Break
11:00 – 11:20 In vivo Imaging of Cell Migration in Zebrafish
Dr Jana Koth
King’s College London
Zebrafish are ideally suited for imaging cell migration in the living organism (in vivo). Their embryos and larvae are small, transparent, develop at room temperature without the need of special climate chambers and offer a wide range of genetic tools. In this talk, different examples of imaging migrating cell populations in zebrafish will be introduced and technical advice on in vivo imaging will be given. Furthermore, current challenges and limitations of in vivo imaging of cell migration in zebrafish will be discussed.
11:20 – 11:40 Image Analysis in 2 and 3D
Blizard Institute of Cell and Molecular Science, UK
Quantification of cell migration is an essential part of the cell biologist’s toolbox. In this talk the theoretical and practical aspects of cell migration will be discussed using Macrophage migration as an example. Different techniques from use of transwell plates to imaging migration in animals will be discussed. How to use some of the image analysis tools which are available for analysing migration will be demonstrated and there will be a discussion of necessary assumptions that need to be made to quantify cell migration.
11:40 – 12:00 TBC
12:00 – 12:30 Working Lunch
Please collect your lunch and take it to your discussion table (Session 1)
12:30 – 13:40 Discussion Groups (Sessions 1, 2, & 3)
· Round table discussion groups (20 minutes each) will be held throughout the afternoon
· Delegates will rotate so that they may participate in all the discussion tables
· All delegates will also be allocated a session for visiting the exhibition stands
· Where appropriate delegates will be able to bring their samples to the discussions
· See end of agenda for description of discussion tables
13:40 – 14:00 Afternoon Talk TBC
14:00 – 15:35 Discussion Groups (Sessions 4, 5, & 6)
15:35 – 16:35 Question and Answer Session
This session will include summing up of the discussion tables and questions submitted both prior to the meeting and throughout the day
16:35 Chairman’s Summing Up and Feedback Prize Draw
Round-table Discussion Sessions:
Table A: High Resolution Imaging in Deep Tissue
Hosted by Rainer Heintzmann, who gained his Diploma in Physics at the University of Osnabrueck in 1996 and in 1999 Ph.D. in Physics from the University of Heidelberg, Germany.From 2000 to 2004, he worked as a scientist in the Max Planck Institute for Biophysical Chemistry in Goettingen, Germany. In addition to heading the Biological Nanoimaging Group at the Randall Division of Cell and Molecular Biophysics at King's College London, he also heads a research division at the Institute of Photonic Technology in Jena. As a Professor of Physical Chemistry he teaches and does research at the Friedrich Schiller University, Jena, Germany. His main research area is high-resolution fluorescence microscopy. He has contributed to the development of a number of microscopy imaging modes such as structured illumination and pointillism. He also has a strong interest in various areas of image processing and is the author of over 40 published journal papers in biomedical imaging.
Table B: Methods to Image Dynamic Adhesions in Cells in 3D Matrices
Hosted by Dr Maddy Parsons, who first became interested in cell adhesion signalling in 3D environments during her PhD studies within the Dept of Medicine at UCL (1996-1999). Her research uncovered a novel role for integrin receptors in controlling ECM deposition in response to external mechanical loads. She then moved onto her postdoctoral position at Cancer Research UK (2000-2005) where she further developed her interests in using complex imaging technqiues to analyse protein interactions and dynamics during cell adhesion and migration. She established her own lab at King's College London in 2005 following award of a Royal Society University Research Fellowship.
Table C: Analysis of Cellular Invasion in 3D Matrix: Imaging and Quantitation
Hosted by Dr Yoshifumi Itoh, a senior lecturer of Matrix Biology at The Kennedy Institute of Rheumatology Division, Imperial College London. His research group studies the mechanism of cellular invasion focusing on the proteinases that degrade extracellular matrix during invasion process in the context of rheumatoid arthritis and cancer. His PhD was carried out at The University of Kansas Medical Center in US (1991-1996), and he was then an assistant professor at The University of Tokyo in Japan (1997-2001). Currently he is also responsible for running live cell imaging lab at the Kennedy institute.
Table D: Technical Advice on in vivo Imaging in Zebrafish
Hosted by Dr Jana Koth, who completed her diploma in animal physiology and zoology at the Humboldt-University in Berlin, Germany in 2006. She undertook her PhD studies at the MRC Centre for Developmental Neurobiology and the Randall Division for Cell & Molecular Biophysics at King’s College London from 2006-2010. For her project she developed in vivoimaging techniques to do long term time-lapse imaging and in vivo cell tracking of developing migrating muscle precursor cells. She is currently working as a post-doc in Simon M. Hughes lab in King’s Randall Division where she did her degree.
Table E: How to Analyse Cell Migration in 2 and 3D
Hosted by Dr Ann Wheeler, who is currently employed as the Manager of the BICMS advanced light microscopy facility (BALM) and is PI of the imaging lab. Previously she has worked at The Scripps Research Insitute analysing behaviour of microtubules in polarising cells. Her PhD was carried out in the Ludwig Institute of Cancer research at UCL where she investigated the role of Rho GTPases in macrophage migration.
Table F: TBC
Hosted by Bitplane
Keywords:
zebrafish, migration, flurescence, confocal, timelapse, MT1-MMP, ECM degradation, invasion, homodimerization
www.regonline.co.uk/workshopLIVECELL2011